【摘要】 目的:寻求一种在液氮条件下冻存猪皮的最佳冷冻保护剂.方法:将新鲜巴马香猪皮肤消毒处理后分为3组,分别用A.20%二甲基亚砜(DMSO)、B.20%二甲基亚砜+6%丙二醇(PG)、C.20%二甲基亚砜+6%丙二醇+0.5 M海藻糖(Trehalose)3种抗冻液作冻存处理后直接投入液氮保存,于15 d、30 d后复温进行活力检测.结果:0.5 mol/L海藻糖与二甲基亚砜、丙二醇联合作为抗冻液所处理的猪皮,复温后其活力明显高于其它两组.结论:海藻糖能有效提高低温储存皮肤的活力,医院在保存皮肤时可以在抗冻液中加入海藻糖以得到较高活力的猪皮用于临床.更多还原

【Abstract】 Objective:to investigate a best cryoprotectant for pigskin storage in liquid nitrogen.Methods:the viability of sample from 3 groups(20%DMSO, 20%DMSO+6%PG,20%DMSO+6%PG+0.5 M Trehalose) was evaluated by MTT.The experiment was carried out 15 days and 30 days.Results:the skin cryopreserved with DMSO/PG/trehalose showed indistinguishable viability from other groups.Conclusion:Cryopreservation protocols for routinely used in hospitals can be improved by combining tehalose.更多还原