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广藿香ITS基因型与地理分布的相关性分析

Correlation between ITS genotype and geographical distribution of Pogostemon cablin

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【Author】 ZHANG Ying 1,2 , CHEN Yao2, ZHANG Jin-chao 3,6 , YANG Meng-su3, CAO Hui4, XIAO Pei-gen 5* (1. School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100029, China; 2. Shenzhen Institute of City University of Hong Kong, Shenzhen 518057, China; 3. Department of Biology and Chemistry, City University of Hong Kong, Hong Kong SAR, China; 4. National Engineering Research Center for Modernization of Traditional Chinese Medicine, Zhuhai 519020, China; 5. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China; 6. College of Chemistry and Environmental Sciences, Hebei University, Baoding 071002, China)

【机构】北京中医药大学中药学院；香港城市大学深圳研究院广东深圳518057；香港城市大学深圳研究院；香港城市大学生物及化学系；河北大学化学与环境科学学院；河北保定071002；国家中药现代化工程技术研究中心；中国医学科学院药用植物研究所；北京100029；广东深圳518057；香港；广东珠海519020；北京100094；

【摘要】探讨广藿香Pogostemoncablin(Blanco)Benth.的地理分布与ITS基因型的关系,为广藿香的道地性分析和种质评价提供分子依据。采用PCR直接测序技术对不同产地的广藿香ITS1和ITS2基因进行测序分析。结果显示,不同产地广藿香ITS1基因和ITS2基因核苷酸序列长度分别为424bp和380bp,其中前者存在19个变异位点,后者存在5个变异位点,说明广藿香的地理分布和其ITS1,ITS2基因型具有良好的相关性,因而ITS1和ITS2测序分析可为广藿香的地理分布与基因型的相关性及其种质评价提供有用的分子信息。更多还原

【Abstract】 To investigate the correlation between genotype and distribution of Pogostemon cablin by sequencing ITS₁ and ITS₂ genes, and provide molecular information for its germplasm evaluation, ITS₁ and ITS₂ genes of Pogostemon cablin from different localities were identified by PCR direct sequencing. The sequences of ITS₁ and ITS₂ genes were 424 bp and 380 bp in length, respectively. And nineteen base substitutions were observed in ITS₁ gene, and five in ITS₂ gene. The results showed a good correlation between genotype and distribution of Pogostemon cablin, and ITS gene sequencing could provide useful molecular information for germplasm evaluation of the plant species verification.更多还原

【关键词】广藿香； DNA测序；种质评价；
【Key words】 Pogostemon cablin； DNA sequencing； germplasm evaluation；

【基金】国家十五攻关计划重大项目(2004BA721A28);香港城市大学应用研究基金(9630002).

【文献出处】药学学报 ,Acta Pharmaceutica Sinica , 编辑部邮箱 ,2007年01期

【分类号】R282
【被引频次】27
【下载频次】452

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