【摘要】 目的:综合考察纳米铁核素(59Fe)的照射剂量率和照射时间对杀死肿瘤细胞的影响,为低剂量率的β粒子放射性核素靶向疗法提供指导。方法:采用的三个肿瘤细胞系为:SKBR-3乳腺癌、U-118MG神经胶质瘤和A-431宫颈癌;实验模型为:在每一个样品中取103个肿瘤细胞,用低剂量率的β粒子照射;初始剂量率为:0.1 Gy/h～0.8 Gy/h;持续照射时间为:1天、3天或者7天。结果:分别用0.2 Gy/h～0.3 Gy/h和0.4 Gy/h～0.6 Gy/h的剂量率连续照射肿瘤细胞7天和3天,能够将所有肿瘤细胞样品中的细胞杀死。细胞的总辐射剂量为30 Gy～40 Gy。而用0.8 Gy/h的剂量率照射24小时后,仅SKBR-3细胞被杀死,其它肿瘤细胞系的所有细胞都能自动修复。结论:在低剂量率的β粒子放射性核素靶向疗法中,该实验结果为照射剂量率和照射时间的确定提供了指导。更多还原

【Abstract】 Objective: The purpose of this study was to identify the effect of different dose rate and expasure time on the tumor cells,which can provide a series of direction for the targeted radionuclide therapy applying low dose rate beta irradiation.Methods: Three tumor cell lines,U-118MG gliomas,A-431 cervical squamous carcinoma and SKBR-3 breast cancer,were used.An experimental model with 103 tumor cells in each sample was irradiated with low dose rate beta particles.The initial dose rates were in the range of 0.1 Gy/h～0.8 Gy/h,and the cells were continuously exposed for 1day,3days or 7days.Results: Continuous irradiation with dose rates of 0.2 Gy/h～0.3 Gy/h and 0.4 Gy/h～0.6 Gy/h for 7days and 3 days,respectively,could kill all cells in all tumor cell samples.The given total radiation doses was 30 Gy～40 Gy.However,when exposed for 24 h with about 0.8 Gy/h,only the SKBR-3 cells were successfully treated and all the other cell types recovered.Conclusions: The results served as a guideline for the combinations of dose rate and exposure time.It is necessary to kill tumor cells that low dose rate beta irradiation should be applied.更多还原