【摘要】 产朊假丝酵母尿酸酶由基因工程菌表达,文章对重组蛋白的亚基相对分子质量、最适作用温度、最适作用pH及其热稳定性和pH稳定性等理化性质进行了考察。成功构建了小鼠高尿酸血症动物模型,并用于重组尿酸酶的初步体内药效学研究。结果:经SDS-PAGE检测重组尿酸酶亚基的表观相对分子质量为32.4 k。酶的最适作用温度和pH分别为40℃和8.0,且在pH6~12,温度20℃~60℃条件下比较稳定。初步体内药效学研究结果表明,重组尿酸酶可以显著降低模型小鼠体内的血尿酸水平。更多还原

【Abstract】 The uricase of Candida utilis was expressed in recombinant Escherichia coli.Its physicochemical properties and pharmacodynamic action was studied.Its subunit molecular weight was analysed by SDS-PAGE.Its optimum temperature and pH value were detected,and its thermal and pH stability were also found.A mouse hyperuricemia model was established and the pharmacodynamic action of the recombinant uricase was studied in vivo on the established model.Molecular weight of the recombinant uricase subunit was 32.4 ku in SDS-PAGE.The recombinant enzyme had the strongest reactive ability in pH 8.0 boric acid buffer at 40℃.It also showed good stability at pH 6～12,and at the temperature 20℃～60℃.The pharmacodynamic experiment showed that the recombinant uricase could greatly reduce the level of uric acid in the model mouse’s serum.更多还原