【摘要】 目的:探讨维生素D受体(VDR)基因FokⅠ位点多态性与汉族人群中重度慢性牙周炎(CP)易感性的关系。方法:搜集汉族中重度牙周炎病人71例,并以50例牙周健康者作为对照组,均采外周静脉血提取基因组DNA,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定FokⅠ基因型,并分析组间基因型和等位基因频率的差异。结果:CP组的基因型分布情况为:FF(24,33.8%),Ff(38,53.5%)和ff(9,12.7%),对照组为:FF(9,18%),Ff(26,52%)及ff(15,30%),组间比较有显著性差异(χ2=7.14,P=0.028)。CP组等位基因频率为F(60.6%),f(39.4%),对照组为F(44%),f(56%),组间比较有显著性差异(χ2=6.48,P=0.011)。FF基因型相对于(Ff+ff)的OR=2.33(95%CI=0.97-5.57);F等位基因相对于f的OR=1.95(95%CI=1.16-3.28)。结论:VDR基因FokⅠ位点多态性与汉族人中重度牙周炎易感性可能存在一定的联系。更多还原

【Abstract】 AIM:To investigate the association of vitamin D receptor gene FokⅠ polymorphism with moderate to severe chronic periodontitis of Han Nationality.METHODS:A restriction fragment length polymorphism(RFLP) for FokⅠ of the VDR gene was analyzed by polymerase chain reaction(PCR),followed by restriction digestion with FokⅠ and gel electrophoresis.The genotypes of 71 moderate severe cases of periodontitis and 50 controls with no periodontitis were analyzed.RESULTS:The genotype distribution in the patient group were 24(33.8%),38(53.5%) and 9(12.7%) and in the control group were 9(18%),26(52%) and 15(30%) for FF,Ff and ff respectively.Allele frequencies were 60.6% and 39.4% for F and f in the patient group;44% and 56% in the control group.Chi square analysis indicated that the distribution of the genotypes and alleles between these two groups were significantly different(P=0.028,P=0.011,respectively).The F allele seemed to increase susceptibility to chronic periodontitis(OR=1.95,95%CI=1.16-3.28).CONCLUSION:It indicated that FokⅠpolymorphism of VDR gene was associated with chronic periodontitis of Han nationality.更多还原