【摘要】 目的:研究胞内信号转导分子LIM矿化蛋白-1在人牙周膜细胞体外矿化过程中的表达。方法:利用组织块法体外培养人牙周膜细胞,实验组培养液中加入矿化诱导剂,对照组不加矿化诱导剂。培养14 d后,免疫细胞化学检测骨涎蛋白的表达;von Kossa染色检测矿化结节的形成。培养期间,采用半定量RT-PCR技术监测LIM矿化蛋白-1、碱性磷酸酶的表达,SPSS11.0软件分析结果。结果:培养14 d,实验组骨涎蛋白免疫细胞化学呈阳性表达,von Kossa染色阳性,矿化结节形成;对照组骨涎蛋白表达阴性,von Kossa染色阴性。RT-PCR结果显示碱性磷酸酶、LIM矿化蛋白-1在实验组和对照组各时段均表达。培养期间,实验组LIM矿化蛋白-1的表达显著高于对照组,其中培养第3 d、14 d时表达分别达到两个高峰,约为对照组的1.4倍、1.5倍。培养期间,碱性磷酸酶的表达显著增高,其中培养14 d时约为对照组的1.5倍。结论:首次发现LIM矿化蛋白-1与人牙周膜细胞的体外矿化过程相关,提示LIM矿化蛋白-1可能在牙周膜细胞的分化、矿化中发挥一定作用。更多还原

【Abstract】 AIM: To investigate the expression pattern of LIM mineralization protein-1 during mineralization of cultured human periodontal ligament cells.METHODS: Human periodontal ligament cells were differentiated via culturing in the presence of dexamethasone,asorbic acid and β-glycerophosphate for bone-like mineralized nodule formation,characterized by von Kossa staining and by immunocytochemistry of bone sialoprotein.Expression of LIM mineralization protein-1 and alkaline phosphatase was analyzed by reverse transcriptase-polymerase chain reaction on day 0,1,2,3,5,7,9,11,14,21 and 28.RESULTS: During the mineralization,LIM mineralization protein-1 and alkaline phosphatase were expressed at every time point,generally held at a statistically significantly higher level compared with the control.For LIM mineralization protein-1,1.4-fold increased on day 3 and 1.5-fold on day 14;for alkaline phosphatase,1.5-fold increased on day 14.CONCLUSION: LIM mineralization protein-1 is found to be involved in the mineralization of human periodontal ligament cells.LIM mineralization protein-1 may play a role in the differentiation and mineralization of human periodontal ligament cells.更多还原