【摘要】 目的:改进复方奎宁注射液中盐酸奎宁和咖啡因含量测定方法。方法:采用高效液相色谱法,在C18柱上,以水-乙腈-三乙胺(790∶205∶5)(用冰醋酸调PH值3.5)为流动相,检测波长为275nm,同时测定复方奎宁注射液中盐酸奎宁和咖啡因的含量。结果:盐酸奎宁在0.546～5.460μg范围内,其峰面积与进样量线性关系良好(r=0.9998),平均回收率为100.7%,RSD=0.7%;咖啡因在0.132～1.328μg范围内,其峰面积与进样量线性关系良好(r=0.9999),平均回收率为100.3%。RSD=0.8%。结论:建立的方法可同时测定复方奎宁注射液中两种成分的含量,较其原有药品标准方法有较大的改进。更多还原

【Abstract】 Objective:To improve the determination method of Quinine Hydrochloride and Caffeine in Compound Quinine Injection.Method:An HPLC method was established to determine the contents of Quinine Hydrochloride and Caffeine in Compound Quinine Injection at the same time on C18 column,by using water-acetonitrile-triethylamine(790∶105∶5)as the mobile phase,and detection wavelength is 275nm.Results:The linear range of Quinine Hydrochloride was 0.546～5.460μg,the recovery of the method was 100.7% with RSD 0.7%;The linear range of Caffeine was 0.132～1.328μg,the recovery of the method was 100.3% with RSD 0.8%.Conclusion:The established method can determine the contents of the two ingredients in Compound Quinine Injection at the same time.This method is an improvement on that of the quality standard of Compound Quinine Injection.更多还原