【摘要】 采用27份不同来源的胡椒属(Piper)材料和1份不同属的草胡椒(Peperomia pellucida)材料用引物OPQ-03扩增得到一条约400碱基对(bp)卡瓦胡椒特异片段。对该片段进行了克隆和序列分析,并根据序列分析结果将上述RAPD分子标记转化为重复性和特异性更好的SCAR(sequence characterized amplifiedre-gions,序列特征化扩增区)分子标记。本研究设计出了1对卡瓦胡椒特异SCAR引物P7.1(5′-GGT CAC CTC ACC GCA GCA GGA TGA ACG-3′)和P7.2(5′-GGT CAC CTC AAT GAC ATG GGA TGA ATC-3′),用这对特异引物对本次试验的28份材料进行PCR扩增,结果只有不同属的草胡椒材料无任何扩增,其它材料均扩增出了预期大小440bp的特异带。更多还原

【Abstract】 This paper studied on 28 pepper materials,including 16 cultivated papper materials,3 wild pepper materials,2 wild relatives of pepper materials,6 kava(Piper methysticum) materials,and 1 Peperomia pellucida materials.According to RAPD analysis,we generate SCAR marker for identifying Kava(Piper,Piperaceae).A Kava-associated fragment with a length ofabout 400 bp was generated with OPQ-03 primer.The fragment was cloned and sequenced.PCR amplification with the specific primers P7.1(5’-GGT CAC CTC ACC GCA GCA GGA TGA ACG-3’) and P7.2(5’-GGT CAC CTC AAT GAC ATG GGA TGA ATC-3’) was performed to 28 materials,which 27 materials amplified the 440 bp specific band except for Peperomia pellucida Kunth.更多还原