【摘要】 研究了衍生化试剂BSTFA对烟草中糖类的衍生化反应,并用GC-MS进行分析.烟草中的糖类用水超声提取,用C18SPE柱预分离,衍生化后用GC-MS分析测定.结果表明,该方法的检出限范围是4.7~7.6 ng,加标回收率为96%~106%,相对标准偏差(RSD)为4.63%~6.06%.实验证明该方法具有较高的准确度、快速和简便的特点.更多还原

【Abstract】 The study researched the derivative way of carbohydrates with BSTFA by GC-MS.We used the way to analysis carbohydrates of tobacco.The carbohydrates of tobacco are extracted by the ultrasonic and beforehand separated by C18 SPE.The carbohydrates are derivatived by BSTFA and then determined by gas chromatography-mass spectrometry.The limits of detection of this method ranged from 4.7~7.6 ng.The recoveries of the method ranged from 96%~106% and the relative standard deviations of peak areas ranged from 4.63%~6.06%.Therefore,the results prove the way is precise、quick and convenient.更多还原