【摘要】 在昆明市文心兰栽培基地发现了具有病毒病症状的感病文心兰和大花惠兰植株。感病文心兰和大花惠兰样品在电镜下可观察到长约460-500 nm的线状病毒粒体和长约300 nm的杆状病毒粒体,它们分别与已报道的建兰花叶病毒和齿兰环斑病毒粒体大小一致。利用ELISA技术在感病文心兰和大花惠兰样品可以检测到建兰花叶病毒和齿兰环斑病毒。利用建兰花叶病毒和齿兰环斑病毒的特异性引物,运用RT-PCR方法可以扩增到与引物设计预期大小一致的核酸条带。由此证明,云南文心兰和大花惠兰病毒病由建兰花叶病毒和齿兰环斑病毒中的一种或两种侵染引起。更多还原

【Abstract】 We find some Oncidium and Cymbidium with symptom of viral disease in Kunming.We discovered 460～500 nm line virus and 300 nm bacilliform virus in plant by electronic microscope.They size accord with Odontoglossum ringspot virus and Cymbidium mosaic virus.Oncidium and Cymbidium with symptom of viral disease affect with ORSV or CyMV by ELISA.Using primer of ORSV and CyMV,we amplified DNA fragments with expected sizes by RT-PCR.Cause of viral disease of Oncidium and Cymbidium is one or two of ORSV or CyMV in Yunnan.更多还原