【摘要】 目的:探讨2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖(2-[(3-carboxy-1-oxoprogy1)amino]-2-deoxy-D-Glucose,COPADG)诱导Eca-109细胞凋亡的机制。方法:不同浓度COPADG作用于人食管癌Eca-109细胞24h,检测Eca-109细胞的抑制率、凋亡率、细胞内活性氧(ROS)、线粒体跨膜电位。结果:Eca-109细胞凋亡率与COPADG浓度呈正相关(rs=1.0,P<0.01);Eca-109细胞线粒体膜电位水平与Eca-109细胞凋亡率相关(rs=1.0,P<0.01);ROS水平与Eca-109细胞凋亡率呈正相关(rs=1.0,P<0.01);ROS水平与Eca-109细胞线粒体膜电位水平呈负相关(rs=1.0,P<0.01)。结论:COPADG可促进Eca-109细胞凋亡,提高Eca-109细胞内ROS水平,并降低线粒体膜电位水平。实验结果提示COPADG提高ROS水平,降低Eca-109细胞线粒体膜电位水平,启动细胞凋亡通路,促使Eca-109细胞凋亡,并且线粒体膜电位的下降是通过提高ROS实现的。更多还原

【Abstract】 AIM: To explore mechanism of apoptosis in Eca-109 cells induced by 2-(3-carboxy-1-oxoprogy1)amino-2-deoxy-D-glucose (COPADG). METHODS: Eca-109 cells were cultured with different concentrations of COPADG for 24 h. Then we examined cell growth inhibitory rate, apoptosis rate, reactive oxygen species (ROS) and ΔΨm of Eca-109 cells. RESULTS: The apoptosis rate of Eca-109 cells and the concentration of COPADG presented a positive correlation (rs=1.0, P<0.01).The ΔΨm and the apoptosis rate of Eca-109 cells were related (rs=1.0, P<0.01).The ROS and the apoptosis rate showed a positive relationship (rs=1.0, P<0.01). The levels of ROS and the ΔΨm were negatively related (rs=1.0, P<0.01). CONCLUSION: COPADG promotes apoptosis of Eca-109 cells, raises ROS level in Eca-109 cells and lows level of ΔΨm. The experimental results suggest COPADG increases ROS level and lows level of ΔΨm triggering the thoroughfare of cell apoptosis to induce apoptosis in Eca-109 cells. The exaltation of ROS results in a decrease in ΔΨm.更多还原