【摘要】 目的:建立用于测定吴茱萸碱血药浓度的液相色谱-串联质谱联用分析方法,并研究吴茱萸碱在大鼠体内的药代动力学。方法:6只大鼠灌胃给药吴茱萸碱100mg/kg,眼底取血,LC-MS/MS法测定血药浓度,并用DAS药代动力学程序拟合计算药代动力学参数。结果:吴茱萸碱浓度在0.2～50ng/mL内,线性关系良好(r2=0.9997)。提取回收率96.12%～99.46%,日内、日间RSD分别为4.61%～13.51%和5.65%～11.49%。主要药代动力学参数为:Cmax=(5.3±1.5)ng/mL;tmax=(22±8)min;t1/2=(451±176)min。结论:建立的LC-MS/MS方法专属性强,灵敏度高,可用于吴茱萸碱的体内定量分析。更多还原

【Abstract】 AIM:To establish an LC-MS/MS method for determination of evodiamine concentration in rat plasma and to study its pharmacokinetic profile in rats.METHODS:Six rats were administrated(i.g.)evodiamine at the dose of 100 mg/kg.Blood samples were collected from eye socket.Evodiamine concentration in rat plasma was determined by LC-MS/MS method.The pharmacokinetic parameters were calculated using DAS program.RESULTS:A good linear relationship was obtained in the concentration range(0.2-50.0 ng/mL)studied(r2=0.9997).Average recoveries ranged from 96.12% to 99.46%.Intra-and inter-day relative standard deviations were 4.61%-13.51% and 5.65%-11.49%,respectively.The main pharmacokinetic parameters of evodiamine were as follows:Cmax(5.3±1.5)ng/mL;tmax(22±8)min;t1/2(451±176)min.CONCLUSION:A selective and sensitive liquid chromatography-tandem mass spectrometric(LC-MS/MS)method for the quantification of evodiamine in rat plasma is developed and validated.This method is successfully applied for the pharmacokinetic studies of evodiamine in rats.更多还原