【摘要】 目的研究正己烷代谢产物2,5-己二酮（2,5-HD）对雪旺细胞神经生长因子（NGF）及其p75神经营养素受体（p75NTR）表达水平的影响。方法取新生Wistar大鼠坐骨神经进行雪旺细胞原代培养,采用差速贴壁法和Thy-1.1抗体进行细胞纯化。采用免疫荧光法观察2,5-HD不同染毒浓度和染毒时间雪旺细胞NGF及其p75NTR表达水平的变化,图像分析软件Image-Pro Plus进行定量分析。结果2,5-HD 5～40 mmol·L^-1染毒24 h可以促进雪旺细胞NGF及其p75NTR的表达,呈浓度-效应关系;2,5-HD 10.0 mmol·L^-1染毒1～48 h,NGF及其p75NTR表达水平呈先增高后降低的趋势。结论2,5-HD可以促进雪旺细胞NGF及其p75NTR的表达,这可能是机体的一种自我保护机制,为使用NGF治疗正己烷中毒性周围神经病提供了依据。更多还原

【Abstract】 AIM To study the effect of 2,5- hexanedione（2,5-HD）,a metabolite of n-hex- ane,on the expressions of nerve growth factor （NGF） and p75 neurotrophin receptor （p75NTR） in Schwann cells.METHODS Primary cultured Schwann cells were obtained from sciatic nerve of newborn rats and purified by method of adhering to plastic in different times and anti-Thy1.1 antibody.The fluoroim- munoassay was used to detect the expressions of NGF and p75NTR and Image-Pro Plus was used to quantify the absorption of cells treated with 2,5-HD.RESULTS Compared with the con- trol group,the expressions of NGF and p75NTR in Schwann cells treated with 2,5-HD 5,10,20 and 40 mmol·L^-1 for 24 h increased in a concentration-effect manner.Treated with 2,5-HD 10 mmol·L^-1 for 1,3,6,12,24 and 48 h,the expressions of NGF and p75NTR in Schwann cells were up-regulated soon,and then down-regulated.CONCLUSION 2,5- HD increases the expressions of NGF and p75NTR in Schwann cells from sciatic nerve of rats,which may be a self-protection mechanism of body and provide scientific evidence for clin- ical therapy of chronic hexane poisoning with NGF.更多还原