【摘要】 目的观察麦冬不同提取部位对H2O2致血管内皮细胞损伤的保护程度是否不同。方法分别用正丁醇、水和氯仿提取麦冬。含终浓度为12.9mg.L-1的各提取部位与H2O2100μmol.L-1共同作用于培养的人脐静脉血管内皮细胞(HUVEC)24h,流式细胞仪测定细胞的凋亡率,并测定上清培养液中超氧化物歧化酶(SOD)活性和脂质过氧化物丙二醛(MDA)含量。结果麦冬3个提取部位均能减少HUVEC凋亡,其中正丁醇提取部位作用更强些。3个部位均能提高细胞培养液中SOD活性;正丁醇和氯仿提取部位能降低MDA含量。结论麦冬正丁醇、水和氯仿提取部位均可不同程度地保护HUVEC。更多还原

【Abstract】 AIM To study whether the degree of protective effects of different extracted fractions from Ophiopogon japonicus(Thunb.) Ker-Gawl. on the damage of vascular endothelial cells injured by H2O2 is different. METHODSThree fractions from Oph. japonicus were extracted by solvents with different polarities, n-butanol, water and chloroform. Human umbilical vascular endothelial cells (HUVEC) were co-incubated with H2O2 100 μmol·L-1 and the extracts 12.9 mg·L-1 for 24 h. Anti-apoptosis effect was observed with flow cytometry. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in HUVEC culture medium were measured. RESULTS All three extracted parts decreased the apoptosis of HUVEC, among which n-butanol extract was the strongest. All three extracted parts increased the SOD activity of HUVEC. n-Butanol extract and chloroform extract decreased MDA content. CONCLUSION The protective efffects of three extracted fractions of Oph. japonicus on HUVEC are at different degrees.更多还原