【摘要】 目的观察榄香烯乳对视网膜母细胞瘤(Rb)HXO-RB44细胞凋亡抑制蛋白survivin表达及端粒酶活性的影响,探讨榄香烯乳对Rb HXO-RB44细胞的作用机制。方法用不同浓度榄香烯乳作用于HXO-RB44细胞,采用四甲基偶氮唑蓝(MTT)比色法测定细胞增生活性;RT-PCR检测survivin mRNA的表达;聚合酶链反应方法检测端粒酶活性。结果榄香烯乳处理后HXO-RB44细胞受到不同程度的抑制,survivin mRNA表达下降;RB细胞系端粒酶活性减弱。结论榄香烯乳诱导HXO-RB44细胞凋亡与survivin有关;可减弱RB细胞系端粒酶活性,影响其周期变化,抑制RB细胞系增生。更多还原

【Abstract】 Objective Retinoblastoma is the most common intraocular malignant neoplasm,and it is a big threat to eyesight and lifespan for children.Chemotherapy has become the most important treatment to retinoblastoma in recent years.Elemene is a natural compound that was isolated from the traditional Chinese medicinal herb,Rhizoma zedoarie.We studied the expression of survivin and telomerase activity in human retinoblastoma HRO-RB44 cell treated with elemene and investigated the mechanism of elemene on HXO-RB44 cells.MethodsThe HXO-RB44 cells were cultured at 37 ℃ in 50 mL/L CO2/air for 24,48 and 72 hours and divided into experimental group and control group.The latter was administered by elemene at the concentrations of 20,40,60,80 and 100 μg/mL.Telomerase activity was detected by PCR,and expression of survivn mRNA was detected using RT-PCR.ResultsThe absorbing value of HXO-RB44 and inhibiting rate of elemene were significant reduced in different time points after treated of various concentrations of elemene in comparison with respective control group(P=0.000).Expression of survivin mRNA decreased by 79%,56%,43% respectively in various concentrations of elemene groups compared with blank control groups at 24 hours(P<0.01).The telomerase activity had not obvious alteration in different concentrations of elemene groups at 24 hours but was significant different between experimental group and blank control group.Elemene could down-regulate the expression of survivin and decreased telomerase activity.ConclusionElemene inhibits human retinoblastoma HXO-RB44 cells by down-regulating the expression of survivin and suppressing telomerase activity in a concentration-dependent manner.更多还原