【摘要】 目的了解P53、GST-π、P170和鼠抗人MDR相关蛋白(MRP)在眼眶腺样囊性癌(ACC)组织中的表达情况。方法免疫组织化学法检测51例眼眶ACC标本和10例正常眼眶组织中P53、GST-π、P170和MRP的表达状况。结果P53、GST-π和P170在眼眶ACC组织中表达阳性率分别为52·94%、100%和82·35%,与眼眶正常组织相比差异有统计学意义。P53和P170在实体型ACC中的表达率明显高于其在腺样-管状型中的表达率;P53在复发组中的表达率明显高于其在初发组中的表达率;各指标之间在表达上无相关性。结论眼眶ACC的多药耐药性与P53、GST-π和P170的过表达有关,但作用机制不尽相同,各指标的检测对于判断预后有参考价值。更多还原

【Abstract】 Objective Adenoid cystic carcinoma(ACC) is a common malignant tumor in the orbit.In the present study,we investigated the expression and clinical significances of the P53,P-glycoprotein (P170),glutathione S-transferase (GST-π) and multidrug resistance associated protein(MRP) in orbital ACC to provide the theoretical information and clinical reference.MethodsFifty-one samples of ACC determined by pathohistology were collected,and 10 normal orbital tissue samples were obtained during the operation for other benign orbital tumor.The expression of P53,GST-π,P170 and MRP in orbital ACC and normal orbital tissues was detected by immunohistochemisry.ResultsP53,GST-π and P170 were expressed in orbital ACC,displaying brown-yellow granule in cellular nuclear,cellular membrane and cytoplasm respectively with a expression percentage 52.94%,100% and 82.35% respectively,showing a higher expression rate in compared with normal orbital tissues(10%,10%,30%, χ2=7.128, 44.546, 10.455, P=0.008, 0.000, 0.001).The positive rates of P53 and P170 expression in adenoid-tubular ACC(30% and 70%)were lower than in solid ACC(85.71% and 100%, χ2=16.648, 10.880, P=0.000, 0.001).The positive rate of P53 expression in recurrent tumor group was higher than in initial tumor group(84.62% versus 42.11%, χ2=7.634, P=0.006);while that of P170 had not significant difference between recurrent tumor group and initial tumor group(P=0.242),and GST-π was strongly expressed in all recurrent tumor,initial tumor,adenoid-tubular ACC and solid ACC samples.No MRP was expressed in both ACC tissue and normal orbital tissue.ConclusionThe hyperexpression of P53,GST-π and P170 participates in the pathogenesis of acquired MDR in orbital ACC by different pathways.These indexes are of great value in the prognosis evaluation of ACC.更多还原