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HPLC测定人参叶提取物中Rg3两种异构体的含量
Determination of Rg3 in The Extract of Folium Ginseng by HPLC
【摘要】 目的:用HPLC测定人参叶提取物中Rg3(R)和Rg3(S)的含量。方法:对人参叶提取物中Rg3进行TLC定性鉴别,并通过HPLC对两种异构体进行含量测定。结果:Rg3(R)在0.70~7.00μg内浓度与峰面积线性关系良好,r=0.9999;Rg3(S)在0.76~7.60μg内浓度与峰面积线性关系良好,r=0.9999。结论:该方法操作简便,结果准确,重现性好,可作为人参叶提取物中Rg3的定量分析方法。
【Abstract】 Objective: To determine the content of Rg3(R) and Rg3(S) in the extract of Folium Ginseng. Methods: Adopting TLC to make sure the quality, and using HPLC to determine the content of two isomers. Results: The linear range of calibration curve was 0.70～7.00μg for Rg3(R),r=0.999 9, and 0.76～7.60μg for Rg3(S),r=0.999 9.Conclusion:The method is simple and accurate, with a good reproducibility, it can be used as an analysis method for this preparation.
- 【文献出处】 中国现代中药 ,Modern Chinese Medicine , 编辑部邮箱 ,2007年03期
- 【分类号】R284
- 【被引频次】2
- 【下载频次】253