【摘要】 目的:建立更年灵胶囊中维生素B1和维生素B6的含量测定方法。方法:采用反相离子对色谱法,以甲醇-庚烷磺酸钠水溶液(15∶85)为流动相,流速为1·0mL·min-1,检测波长为270nm,柱温:35℃,维生素B1和维生素B6理论板数大于4000。结果:维生素B6在0·258~1·935μg呈良好的线性关系,平均加样回收率为97·74%,RSD=1·11%;维生素B1在0·104~0·78μg呈良好的线性关系,平均加样回收率为98·32%,RSD=0·87%。结论:此方法快速,结果准确,操作简便,可用于含有微量维生素B1、B6的中药制剂产品质量研究。更多还原

【Abstract】 Objective: To establish a RP-HPLC method to determine the content of Vitamin B₁ and Vitamin B₆ in Gengnianling capsules. Methods: The sample was determined by RP-HPLC. The mobile phase consisted of methanol-sodium sulfonic acid heptane solution（15∶85）. The flow rate was 1.0mL·min -1 . The detection wavelength was at 270nm. The temperature of the column was 35℃. The number of theoretical plates of Vitamin B₁ and Vitamin B₆ are both above 4 000. Results: The Vitamin B₆ calibration curve was linear in the range of 0.258～1.935μg, the average recovery rate was 97.74%, the RSD was 1.11%. The Vitamin B₁ calibration curve was linear in the range of 0.104～0.78μg, the average recovery rate was 98.32%, the RSD was 0.87% .Conclusion:The RP-HPLC method for determination Vitamin B₁ and Vitamin B₆ is speediness, accurate, simple and suitable for quality studies of Chinese traditional medicine which contains little Vitamin B₁ and Vitamin B₆.更多还原