【摘要】 目的探讨细胞间黏附分子1(ICAM-1)和血管细胞间黏附分子1(VCAM-1)在急性白血病(AL)中的表达及临床意义。方法39例初诊AL患者,其中27例经治疗后达完全缓解(CR)。采用逆转录-PCR法检测AL骨髓单个核细胞ICAM-1和VCAM-1的 mRNA表达水平。对照组12例均为骨髓像正常的门诊患者。结果ICAM-1和VCAM-1水平在初诊AL者明显高于CR组及对照组(P<0.01),且CR组和对照组之间无显著差异(P>0.05);高白细胞(WBC>100×109/L)组和死亡组的ICAM-1和VCAM-1水平明显增高(P<0.05)。髓外浸润组仅ICAM-1高于非髓外浸润组(P<0.01);非淋巴细胞AL(ANLL)具有良好核型者ICAM-1水平低于其他患者(P<0.05)。结论AL表达ICAM-1和VCAM-1明显增高,其检测对疗效及预后判断有重要意义。更多还原

【Abstract】 Objective To investigate the expression and its significance of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) in acute leukemia (AL). Methods The expressions of ICAM-1 and VCAM-1 were detected by reverse transcriptase-PCR analysis in bone marrow mononuclear cells of 39 untreated AL. Twenty-seven of 39 patients achieved complete remission (CR) after chemotherapy.Twelve out-patients with normal bone marrow were taken as the controls. Results The expressions of ICAM-1 and VCAM-1 in untreated group were much higher than those in the control and CR group (P<0.01), which were not significantly different between CR group and control group (P>0.05).Elevated levels of ICAM-1 and VCAM-1 were seen in hyperleukocyte group (WBC>100 109/L) and death group(P<0.05). The untreated patients with extramedullary invasion showed higher expression of ICAM-1,but not VCAM-1,than those without extramedullary invasion (P<0.01).The untreated ANLL patients with good prognostic karyotype showed lower expression of ICAM-1 than other patients (P<0.05). ConclusionThe expressions of ICAM-1 and VCAM-1 were abnormal in AL, which may be taken as a useful marker for evaluating the therapeutic efficacy and prognosis.更多还原