【摘要】 目的探讨血小板特异性抗体与网织血小板(RP)、血小板相关抗体(PAIgG)在特发性血小板减少性紫癜(ITP)诊断中的价值。方法通过改良的单克隆抗体特异性捕获血小板抗原试验(MAIPA)测定ITP(86例)与非免疫性血小板减少(30例)患者血浆中血小板膜糖蛋白(GP)Ib、GPⅡb-Ⅲa以及P-选择素自身抗体,同时以流式细胞术(FCM)检测相应的RP%及PAIgG。结果ITP组MAIPA、RP%和PAIgG检测阳性率分别为62·79%、80·23%和70·93%;非免疫性血小板减少组分别为6·67%、23·33%和36·67%;两组间差异显著(P<0·01)。三种方法检测ITP结果具有相关性(P<0·05)。结论MAIPA是ITP的特异性诊断指标,多种抗体应用可以提高其敏感度。MAI-PA联合PAIgG及RP测定有益于ITP的正确诊断。更多还原

【Abstract】 Objective To investigate the diagnosis value of platelet glycoprotein-specific autoantibody, reticulated platelet(RP) and platelet-associated immunoglobulin(PAIgG) in the patients with idiopathic thrombocytopenic purpura(ITP).Methods By a monoclonal antibody immobilization of platelet antigen assay(MAIPA),antiplatelet autoantibodies were detected using monoclonal antibodies specific for glycoproteins(GP) Ⅰb,Ⅱb/Ⅲa and P-selectin in patients with ITP and nonimmune thrombocytopenia.Meanwhile,RP% and PAIgG were measured in the same samples with flowcytometry(FCM).Results The positive rates of MAIPA,RP% and PAIgG were 62.79%,80.23% and 70.93% respectively in patients with ITP,which were markedly higher than those in patients with nonimmune thrombocytopenia(NITP)(6.67%,23.33% and 36.67%,respectively)(P<0.01).Significant correlations existed among MAIPA,RP% and PAIgG assays(P<0.05).Conclusion MAIPA is a specific method for the diagnosis of ITP.The sensitivity of MAIPA can be increased by application of many kinds of antibodies.A correct diagnosis of ITP may achieved by combined measurement of MAIPA with RP and PAIgG.更多还原