【摘要】 目的研究大孔吸附树脂分离纯化鬼针草总黄酮的工艺。方法以鬼针草总黄酮吸附量、总黄酮含量和总黄酮回收率为考察指标,从吸附分离特性对树脂进行筛选和工艺优化。结果HPD100树脂对鬼针草总黄酮有良好的吸附分离性能,其吸附分离鬼针草总黄酮的工艺条件为:原液上样浓度为1~1.5g/L,调pH为4,以2BV/h的吸附速率进行吸附,最大上样量以总黄酮计为4.99g/L树脂。吸附后先以4BV水洗去杂质,再用4BV的70%乙醇以4BV/h的速率进行洗脱。在此条件下进行吸附、洗脱,树脂可重复使用5次。结论HPD100树脂在所确定的工艺条件下,可较好的吸附分离鬼针草总黄酮。更多还原

【Abstract】 Objective To study the purification technology of total flavonoids from Bidens bipinnata L.(TFB) with macroporous resins.Methods The adsorption quantity,the content and recovery rate of total flavonoids were used as the evaluating criteria,and the macroporous resins were screened and optimized from the adsorption and desorption property.Results The HPD100 resin had the best separating efficiency when the concentration of the extraction sample was 1~1.5 g/L,the pH value of the sample was 4,the maximum adsorbing capacity for the total flavonoids of Bidens bipinnata L.was 4.99 g/L with the adsorption velocity 2BV/h,decontamination with 4BV distilled water then 70% ethanol was used to elute the total flavonoids,the eluting volume was 4BV and the eluting velocity was 4 BV/h.On this condition to adsorption and elution,the HPD100 resin could be used 5 times repeatedly.Conclusion The HPD100 resin can be used to purify TFB.更多还原