【摘要】 目的检测从临床标本中分离的146株变形杆菌对14种抗菌药物的体外活性以及产AmpC酶情况。方法药敏检测采用M-H肉汤微量稀释法,对头孢西丁耐药株以改良三维试验检测其产AmpC酶情况。PCR法检测AmpC基因型别,并进行了DNA测序分析。结果亚胺培南、氨苄西林/舒巴坦、哌拉西林/他唑巴坦、阿米卡星、氨曲南和三代、四代头孢菌素敏感率>80%。改良三维试验检测出单产AmpC酶奇异变形杆菌2株,检出率为1.4%(2/146)。DNA测序结果发现,2株变形杆菌所产AmpC酶均为CMY-2,基因序列已在基因库注册,授权号:EF534292。结论在变形杆菌中发现2株携带AmpC酶,其基因型为cmy-2。亚胺培南、氨苄西林/舒巴坦、哌拉西林/他唑巴坦、阿米卡星、氨曲南和三代、四代头孢菌素可作为治疗变形杆菌感染的首选药物。更多还原

【Abstract】 Objective To investigate the distribution of AmpC gene in the clinical proteus, detect the activity of 14 kind of antibiotics against 146 strains of proteus isolated so as to guide the rational application of antibiotics.Meth-ods Antimicrobial susceptibility was tested by M-H broth microdilution. Modified three-dimensional extract testwas adopted to detect theβ-lactamase phenotype. The genotype of AmpCβ-lactamase was determined by PCR andDNA sequencing.Results The rate of producing AmpCβ-lactamase was 1.4%(2/146).Of the 146 proteus,cmy-2 AmpC gene was found in2 clinical isolates.The gene was submitted to the GenBank database. accession number:EF534292.The sensitive rate of proteus clinical isolates to imipenem,ampicillin-sulbactam,piperacillin-tazobactam,amikacin,aztreonam, third-generation cephalosporins, fourth-generation cephalosporins were more than 80%.Con-clusion Third-generation cephalosporins,fourth-generation cephalosporins,aztreonam, imipenem and amikacin arethe first candidates for the infectious conditions caused by the proteus.CMY-2 AmpCβ-lactamase is found in 2 P.mirabilis.更多还原