【摘要】 采用序列特异性引物-聚合酶链式反应(PCR-SSP)为基础的人类血小板抗原(HPA)基因分型技术做群体调查,在1,000例受检者中发现1例罕见的HPA-10w(a+b+)杂合子个体,为了验证分型的可靠性,使用PCR反应特异性扩增HPA-10基因片段,然后测序分析。结果表明,nt263位G→A导致GPⅢa糖蛋白第62位精氨酸(CGA)→谷氨酰胺(CAA),产生HPA-10bw抗原特异性。在中国人群中检测出HPA-10bw低频抗原,提示在血小板同种免疫引起的新生儿同种免疫血小板减少症(NAIT)、输血后紫癜症(PTP)以及血小板输注无效症(PTR)的诊断中,该抗原具有临床意义。更多还原

【Abstract】 A total of 1,000 Chinese blood donors were typed for human platelet antigens(HPA) using a sequence specific primers-polymerase chain reaction(PCR-SSP) based HPA genotyping method.An individual with a rare HPA-10w(a+b+) genotype was found.In order to confirm the typing results,a fragment of HPA-10 gene was amplified by PCR and then sequenced.Sequencing data showed that a single G to A substitution at nucleotide 263 occurred,resulting in amino acid change from Arg(CGA) to Gln(CAA) at position 62 of GPⅢa protein.The substitution generated antigenic specificity HPA-10bw.The detection of an HPA-10bw allele in the Chinese population suggests that this rare allele should be consid-ered in platelet alloimmunization,such as neonatal alloimmune thrombocytopenia(NAIT),post-transfusion thrombocyto-penic purpura(PTP) and post-transfusion refractoriness to platelets(PTR).更多还原