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半重叠式多重引物PCR和基因扫描技术在T细胞淋巴瘤诊断中的应用

Detection of T cell receptor γ gene rearrangement in paraffin-embedded tissues of lymphoma by semi-nested multicolor multiplex PCR and Gene-Scan

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【作者】 金贵善全成旭刘福生柴奇

【Author】 JIN Gui-shan1,QUAN Cheng-xu2,LIU Fu-sheng1,CAI Qi1(1.Beijing Neurosurgical Institute,Capital University of Medical Sciences,Beijing 100050,China;2.Yanbian University College of Basic Medicine, Yanji 133000,Jilin,China)

【机构】 北京市神经外科研究所首都医科大学延边大学基础医学院北京市神经外科研究所首都医科大学 北京100050吉林延吉133000北京100050

【摘要】 [目的]探讨半重叠式多重荧光多重引物PCR和基因扫描技术在T细胞淋巴瘤诊断中的应用.[方法]从普通石蜡切片组织标本中提取基因组DNA,用半重叠式多重荧光多重引物PCR扩增,利用DNA测序仪对其产物进行基因扫描分析.[结果]37例T细胞淋巴瘤中27例检测出有克隆性重排;25例B细胞淋巴瘤中2例呈阳性;10例何杰金氏淋巴瘤和7例反应性增生病例均呈阴性.[结论]在T细胞受体γ基因重排克隆性检测中,半重叠式多重荧光多重引物PCR及基因扫描技术具有样本来源便利,灵敏度高,特异性强,耗时短等优点,可作为T细胞淋巴瘤的诊断及鉴别诊断的辅助检测手段.

【Abstract】 OBJECTIVE To explore the application of semi-nested multicolor multiplex PCR and Gene-Scan in the detection of T cell receptor γ(TCR-γ) rearrangement.METHODS DNA from paraffin-embedded tissues were extracted,and amplified by semi-nested multicolor multiplex PCR.The fluorescence labeled PCR products were separated with an automated sequencing system,and analyzed by Gene-Scan.RESULTS 27 out of 37 cases of T-cell lymphoma and 2 out of 25 cases of B-cell lymphoma were positive for clonal TCR-γ rearrangement,10 cases of Hodgkin lymphoma and 7 cases of reactive lymphoid hyperplasia were negative.These results were corresponding to the detection by southern blot.CONCLUSION Semi-nested multicolor multiplex PCR-Gene-Scan technique is a sensitive and convenient tool for TCR-γ rearrangement analysis,and can be useful in diagnosis and differential diagnosis of T cell lymphoma.

  • 【文献出处】 延边大学医学学报 ,Journal of Medical Science Yanbian University , 编辑部邮箱 ,2007年02期
  • 【分类号】R733.1
  • 【下载频次】93
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