【摘要】 目的:研究卡介菌多糖核酸（BCG-PSN）对尖锐湿疣（CA）病人外周血CD8q+T细胞内白细胞介素类（IL-2、IL-4、IL-12）和干扰素γ（IFN-γ）表达的影响。方法:60例CA病人分为2组,均采用CO₂激光去除疣体。BCG-PSN组40例,激光术后开始加用BCG-PSN 1.0mg,im,qod,连续18次,36d为一个疗程。病例对照组20例,激光术后未再用任何药物治疗。另设正常对照组20例。采用流式细胞仪测定3组外周血CD8+T细胞IL-2、IL-4、IL-12、IFN-γ的水平。结果:治疗前CA病人外周血IL-2、IL-12和IFN-γ阳性CD8+T细胞百分率显著低于正常对照组,Tcl/Tc2亦显著低于正常对照组（P<0.01）。治疗后BCG-PSN组外周血IL-2、IL-12和IFN-γ阳性CD8+T细胞百分率显著升高（P<0.01）,病例对照组无显著变化（P>0.05）,2组变化差异非常显著（P<0.01）。BCG-PSN组治愈率82％,显著高于病例对照组（45％,P<0.01）;复发率18％,低于病例对照组（55％,P<0.01）。结论:CA病人存在Tcl/Tc2失调,BCG-PSN可通过上调CA病人外周血CD8+T细胞IL-2、IL-12、IFN-γ的表达,纠正机体细胞因子失调而提高CA病人临床疗效、减少其复发。更多还原

【Abstract】 AIM:To investigate the effects of polysaccharide nucleic acid fraction of Bacillus Calemette- Guerin（BCG-PSN）on the cytokines（IL-2,IL-4,IL-12 and IFN-γ）of CD8+T lymphocytes in patients with condyloma acuminatum（CA）.METHODS:Flow cytometric analysis was employed to study intracellular cytokines（IL-2,IL-4,IL-12 and IFN-γ）of CD8+T lymphocytes in peripheral blood of 60 patients with CA （40 patients treated with CO₂ laser plus BCG-PSN,1.0mg,im,qod,the course lasted for 36d;20 patients treated with CO₂ laser only）and those of 20 healthy controls.RESULTS:Before treatment,the percentage of IL-2-producing,IL-12-producing and IFN-γ-producing CD8+T lymphocytes were significantly lower（P<0.01）, while the ratio of Tc1/Tc2 was lower in CA patients than those in healthy controls（P<0.01）.The percentages of IL-2-producing,IL-12-producing and IFN-γ-producing CD8+T lymphocytes significantly increased（P<0.01）, and the ratio of Tcl/Tc2 returned to normal in CA patients after treatment with BCG-PSN.The percentages of IL-2 -producing,IL-12-producing and IFN-γ-producing CD8+T lymphocytes showed no significant changes in case- control group before and after treatment（P>0.05）.The clinical cure rate in BCG-PSN group was significantly higher than that of case-control group（82% vs 45%,P<0.01）;the recurrent rate in BCG-PSN group was significantly lower than that of case-control group（18% vs 55%,P<0.01）.CONCLUSION:There are Tc1/Tc2 imbalance in the peripheral blood of CA patients.Treatment with BCG-PSN can induce effectively the levels of IL-2,IL-12 and IFN-γin the CD8+T lymphocytes,regulating the Tc1/Tc2 imbalance and in terms can significantly increase the clinical cure rate,and decrease the recurrent rate in CA patients.更多还原