【摘要】 目的:以不同分子质量的壳聚糖为原料合成系列的N-正辛基-N′-琥珀酰基壳聚糖,考察其与肿瘤细胞的亲和性,为其作为抗肿瘤药物的靶向载体提供依据。方法:用异硫氰酸荧光素(FITC)对N-正辛基-N′-琥珀酰基壳聚糖进行标记,再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞(BGC)共培养,通过流式细胞仪及酶联免疫检测仪测定N-正辛基-N′-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。结果:N-正辛基-N′-琥珀酰基壳聚糖对4种肿瘤细胞亲和性明显强于空白细胞株(P＜0．01),并具有一定的肿瘤抑制作用。结论:N-正辛基-N′-琥珀酰基壳聚糖有望作为抗肿瘤药物的靶向载体。更多还原

【Abstract】 AIM:To study the affinity with tumor cells through series of N-octyl-N′-succinyl chitosan synthesized by using different moleeular weight of chitosan.METHODS:A series of N-octyl-N′-succinyl chitosan labeled with fluoreseein(FITC),were incubated with HepG2 liver cancer cells,K562 leukemia cells, A549 human lung cancer cells,and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-oetyl-N′-succinyl ehitosan for the four kinds of tumor cells already mentioned.RESULTS:N-octyl-N′-sueeinyl ehitosan possessed stronger affinity than control cell lines and growth inhibition with the four kinds of tumor cells.CONCLUSION:N-octyl-N′- succinyl chitosan may possibly be a tumor-targeting carrier.更多还原