【摘要】 目的:研究黄芪注射液对脂多糖所致大鼠急性肺损伤（ALI）后细胞凋亡的保护作用,探讨其可能的机制。方法:雄性Wistar大鼠200～250 g 45只,随机分成3组,NS对照组、ALI组和黄芪注射液组。后2组以颈外静脉注射脂多糖5 mg·kg^-1致急性肺损伤。分别观察各组呼吸频率、动脉血氧分压（PaO₂）,处死后检测肺组织湿/干（W/D）比值,免疫组化法检测肺泡上皮细胞Bcl-2、Bax蛋白表达。结果:NS对照组呼吸频率为（73±s 6）次·min^-1,PaO₂为（90．0±2．0）mmHg,W/D比值为3．287±0．024, Bax/Bcl-2为0．5±1．0;ALI组与NS对照组相比,呼吸频率增快为（112±6）次·min^-1（P＜0．01）,PaO₂降低为（46±3）mmHg（P＜0．01）,W/D比值升高为5．151±0．023（P＜0．01）,Bax/Bcl-2上升为7．8±0．7（P＜0．01）;黄芪注射液组与ALI组相比,呼吸频率减慢为（94±4）次·min^-1（P＜0．01）,PaO₂上升为（84±6）mmHg（P＜0．01）,W/D比值降低为4．809±0．014（P＜0．01）,Bax/Bcl-2下降为0．1±0．7 （P＜0．01）。结论:黄芪注射液可能通过降低Bax/Bcl-2细胞比例抑制细胞凋亡,减轻脂多糖所致ALI。更多还原

【Abstract】 AIM:To observe the protective effects and the mechanism of astragali injection in rats with lipopolysaccharide-induced acute lung injury（ALI）.METHODS:Forty-five rats were randomly divided into three groups:control group,ALI group,and astragali injection group.The respiratory rates and partial pressure of arterial oxygen were measured.The rats were killed and the ratios of wet/dry lung tissue were detected.The Bcl-2-positive cells and Bax-positive ceils were measured using immunocytochemistry in all groups.RESULTS: In control group,the respiratory rate was（73±s 6）min^-1,partial pressure of arterial oxygen（PaO₂）was （90.0±2.0）mmHg,the ratio of wet/dry lung tissue was 3.287±0.024,and Bax/Bcl-2 was 0.5±1.0.Com- pared with control group,the respiratory rate,the ratio of W/D of lung tissue,and Bax/Bcl-2 in ALI group in- creased to（112±6）min^-1,5.151±0.023,and 7.8±0.7,with P＜0.01 respectively,and PaO₂ decreased to（46±3）mmHg（P＜0.01）.Compared with ALI group,the respiratory rate,the ratio of wet/dry lung tis- sue,and Bax/Bcl-2 in ALl group decreased to（94±4）min_-1,4.809±0.014,and 0.1±0.7,with P＜0.01 respectively,and PaO₂ increased to（84±6）mmHg（P＜0.01）.CONCLUSION:Astragali injection has protective effects through inhibiting the apoptosis of the pulmonary epithelium.更多还原