【摘要】 目的:探讨高危型人乳头瘤病毒(HR-HPV)检测在宫颈病变筛查中的应用价值。方法:应用第二代杂交捕获法(HC-Ⅱ)和液基薄层细胞学(TCT)对839例受检者同时行13种高危型HPV DNA检测和宫颈细胞学检查;HPV阳性和细胞学结果为非典型鳞状细胞(ASCUS)及以上患者行阴道镜下病理活检,分析HPV感染与宫颈上皮内病变的关系。结果:HPV检出率随宫颈病变程度加重呈上升趋势,ASCUS中HPV检测阳性率为75.47%,HPV阳性的ASCUS患者中病理活检47.17%为宫颈上皮内瘤变或更严重病变。结论:HPV检测在宫颈病变筛查中可作为细胞学的辅助手段提高诊断准确性,并可指导HPV感染者的治疗及用于患者的追踪随访。更多还原

【Abstract】 Objective:To investigate the application value of high-risk human papilloma virus (HR-HPV) DNA test in cervical lesions screening. Methods:839 cases cytological specimens exfoliated from cervix were tested by both the hybrid capture Ⅱ (HC-Ⅱ) assay and the thin prep pap smear fluid-based system (TCT) for detecting 13 types of high-risk HPV DNA. Both patients with positive results of high-risk HPV DNA and pap smear showed atypical squamous cells of undetermined significance (ASCUS) were performed colposcopy and histological examination on the cervical tissue. Results:HPV infection rate increased according to the seriousness degree of cervical lesions. Of the patients with ASCUS and positive results of high-risk HPV DNA,47.17% of patients were found to have cervical intraepithelial neoplasm (CIN). Conclusion:HPV DNA test is very important to improve the diagnostic accuracy. Earlier detection of high-risk HPV types can instruct the treatment and trace the infected patients.更多还原