【摘要】 为了研究干扰素α对白血病细胞U937細胞的抗增殖作用及其机制,用不同浓度的干扰素α(500U/L、1000U/L、2000U/L、3000U/L、4000U/L)对U937細胞作用不同时间(0、12、24、36和48小时),用MTT法检测细胞生长抑制率,应用流式细胞术(FCM)检测细胞凋亡率,RT-PCR方法分析cyclinEmRNA的表达。结果表明:不同浓度的干扰素α处理细胞后,U937细胞生长明显受抑,2000U/L干扰素α能引起细胞凋亡,凋亡率为25.28%-70.54%(P<0.01),细胞周期相关基因cyclinEmRNA表达降低;干扰素α对U937细胞的抑制率,呈浓度-时间依赖性。结论:干扰素α对U937细胞有明显抗增殖和促凋亡作用,此结果将为干扰素-α在白血病临床治疗方面的应用提供有力的实验证据。更多还原

【Abstract】 This study was aimed to investigate the anti-proliferation effect of interferon-α(IFN-α) on leukemic U937 cells and its mechanism.The U937 cells were given with various concentrations of IFN-α(500, 1 000, 2 000, 3 000 and 4 000 U/L) and at different time (0, 12, 24, 36, 48 hours), the inhibitory ratio was measured by MTT assay, apoptosis rate was detected by flow cytometry(FCM), the expression of cell cycle-associated cyclin E mRNA was measured by RT-PCR. The results showed that IFN-α (2 000 U/L) could cause apoptosis, after being treated by various concentrations of IFN-α, the growth of U937 cells was inhibited significantly, the apoptosis rate was 25.82%-70.54% (P<0.01), the cycle-associated cyclin E mRNA expression decreased, the growth of U937 cells was significantly inhibited, the suppression of U937 by IFN-α was both in time-and dose-dependent manner.It is concluded that IFN-α has apparent anti-proliferation and apoptosis-inducing effects on U937 cells. These results will provide strong laboratory evidence for IFN-α clinical application in leukemia therapy.更多还原