【摘要】 目的探讨HSV-tk/GCV系统基因治疗膀胱癌细胞的方法及结果。方法与结果采用了带有CMV启动子驱动HSV-tk基因的重组腺病毒载体,体外转染HTB9细胞后对GCV敏感性增强。当MOI=100时,HTB9细胞的IC50为2.5mg/L,病毒浓度降低,GCV对细胞的杀伤效率亦降低。将不同比例的tk+细胞和tk-细胞混合后加入10mg/L GCV,发现HSV-tk/GCV系统有很强的“旁观者效应”。结论HSV-tk/GCV系统是一种有效、安全地治疗膀胱癌的新方法。更多还原

【Abstract】 Objective To study the new way of adenovirus-mediated gene therapy for bladder cancer.Methods and Results We used the replication-defective adenovirus carring the HSV-tk gene(Ad.tk) under control of the cytomegalovirus(CMV).In vitro experiments,the HTB9 cells transferred by tk gene were killed in the presence of GCV treatment.When tk+ cells were mixed with their parental cells at various ratios,GCV not only killed tk+ cells,but neighboring cells without tk gene also killed by bystander effect.Conclusion Adenovirus-mediated HSV-tk/ GCV system may be used as apromising suicide gene therapy for bladder carcinoma.更多还原