【摘要】 目的建立春雨烧伤凝胶剂中盐酸小檗碱含量测定的高效液相色谱法。方法色谱柱为Kromasil C18柱(4.6mm×250mm,5μm),流动相为乙腈-1%H3PO4-三乙胺(24∶76∶0.76),检测波长:345nm,流速:1.0mL/min,柱温:30℃。结果盐酸小檗碱在0.088～0.440μg(r=0.9997)之间具有良好的线性关系,平均回收率为98.13%,RSD=0.77%(n=6)。结论该法检测快速,精密度高,重现性好,可用于春雨烧伤凝胶剂的定量分析。更多还原

【Abstract】 Objective To establish an HPLC method for determination the contents of berberine hydrochloride in spring rain burns gels. Methods Analytical column was Kromasil C18 (4.6 mm×250 mm, 5 μm) column with a mobile phase of Acetonitrile-1%H3PO4-Triethylamine (24∶76∶0.76), the detective wavelength was 345 nm, the flow rate was 1.0 mL/min and column temperature was 30 ℃. Results A good linearity was obtained in the range of 0.088～0.440 μg (r =0.999 7), and the average recovery was 98.13% with RSD=0.77% (n=6). Conclusion The method is rapid and reliable, and can be used for the quality control of spring rain burns gels.更多还原