【摘要】 目的采用高效液相色谱法测定金茵颗粒剂中绿原酸的含量。方法Kroasil ODS柱(4.6mm×250mm);柱温:室温;流动相:0.3%乙酸-甲醇(74∶26);流速:0.9mL/min;检测波长:327nm。结果该方法的线性范围为65～325μg/mL,回归方程为:A=24239869C-31292,r=0.9993;平均回收率为100.10%,RSD=0.87%(n=6)。结论该方法可作为金茵颗粒剂的质量控制方法。更多还原

【Abstract】 Objective To establish a HPLC method to determind the content of chlorogenic acid in Jinyin Granules. Method The mobile phase was 0.3%HAc-Methonal(74∶26). The UV detection wavelength was 327 nm. The mobile speed was 0.9 mL/min. Separation column was Kroasil ODS(4.6 mm×250 mm). Result A good linearity was obtained in the range of 65～325 μg,regression A=24 239 869C-31 292,r=0.999 3. The average recovery was 100.10%,RSD was 0.87%(n=6). Conclusion The method was good for determining chlorogenic acid in Jinyin Granules.更多还原