【摘要】 目的探讨邻苯二甲酸二丁酯对肝细胞的毒性作用及可能的损伤机制。方法1、10、50、100μmol/L浓度的邻苯二甲酸二丁酯作用于人肝细胞HL-7702后,检测邻苯二甲酸二丁酯对肝细胞HL-7702的增殖率、超氧化物歧化酶(SOD)、丙二醛(MDA)含量的变化。结果邻苯二甲酸二丁酯(DBP)作用于肝细胞后,肝细胞HL-7702的增殖率随DBP浓度的增加而下降,在≥10μmol/L浓度,细胞的增殖率显著降低(P<0.01),肝细胞的增殖率从对照组的100%降低到100μmol/L浓度的50.7%。DBP可使肝细胞HL-7702中的SOD含量降低,MDA含量升高,当浓度大于50μmol/L时,与对照组相比均具有统计学意义(P<0.01)。结论DBP对体外培养人肝细胞HL-7702具有细胞毒性和氧化损伤作用。更多还原

【Abstract】 Objective To study the toxic effects of Di-n-butyl phthalate on human liver HL-7702 cells and the possible mechanisms.Methods Human liver HL-7702 cells were exposed to different concentration of Di-n-butyl phthalate(1μmol/L,10mol/l,50mol/L,100mol/L).Proliferation rate and level of SOD,MDA were measured.Results After exposed to Di-n-butyl phthalate,HL-7702 cell proliferation rate decreased when the concentration of Di-n-butyl phthalate increased.Din-butyl phthalate significantly decreased HL-7702 cell proliferation rate at 10mol/L,Human Liver proliferation rate from 100% of contrast group to 50.7% of 100mol/L.Di-n-butyl phthalate significantly decreased SOD level and increased MDA level at 50mol/L.Conclusion Di-n-butyl phthalate has cytotoxic effect and oxidative damage to HL-7702 cells.更多还原