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草莓离体培养中玻璃化的发生及克服措施研究
Vitrification in in Vitro Culture of Strawberry and Its Prevention
【摘要】 以“丰香”品种为试材,MS为基本培养基,研究草莓玻璃化的克服措施,结果表明,在6-BA试验浓度范围内(1-3 mg.L-1),玻璃化率随6-BA浓度的增加而增加;40 g.L-1蔗糖,降低MS基本培养基中铵离子浓度,加入活性炭,采用透气膜封口在一定程度上均能使玻璃苗恢复正常.
【Abstract】 Cultured in vitro on MS medium supplemented with 6-BA,the strawberry variety "Tochiotome" showed increasing vitrification rate with increasing 6-BA concentration(1~3 mg/L).The addition of sucrose to the medium at 40 g/L helped to decrease the number of vitreous plantlets.The measures of decreasing NH+4 concentration in the medium,or adding active carbon to it,or sealing the bottle with a permeable film helped the vitreous plantlets change back to normal ones.
【基金】 广东省农业厅农业科技项目(B06026)
- 【文献出处】 西南大学学报(自然科学版) ,Journal of Southwest University(Natural Science Edition) , 编辑部邮箱 ,2007年04期
- 【分类号】S668.4
- 【被引频次】44
- 【下载频次】348