【摘要】 为了阐明氯氰菊酯(CP)影响睾酮合成的机制,本研究以成年SPF昆白小鼠为试验动物,通过连续7 d灌喂不同浓度的高效氯氰菊酯(β-CP),采用RT-PCR、Western blot和放射免疫分析技术分析了β-CP对睾丸重量、血浆类固醇水平、类固醇合成酶、StAR蛋白以及ERK1/2活性的影响。结果显示:随着β-CP浓度的增加,小鼠睾丸的重量无明显变化,但小鼠血浆睾酮的水平逐渐降低;P450sccmRNA、3β-HSDmRNA和StAR mRNA的水平则无明显变化,而P450 c17 mRNA的表达明显下降;StAR蛋白的水平也呈下降趋势,但ERK1/2活性则逐渐升高。以上结果表明,β-CP通过激活ERK1/2而级联抑制P450 c 17 mRNA的转录和StAR前体蛋白的裂解,从而降低睾酮的合成。更多还原

【Abstract】 To study the effects of β-cypermethrin on the testosterone synthesis.Matured kunbai mouse were fed different concerntration of β-cypermethrin for 7 days.RT-PCR,Western blot and radioimmunoassay(RIA) were used to analyze the ability of testosterone synthesis.The results were as followed: with the increase of β-cypermethrin concerntration,the weight of testis,the expression of P450scc mRNA,3β-HSD mRNA and StAR mRNA had no difference between different groups.However,the expression of P450c17 mRNA and the level of StAR protein decreased significantly and the activity of ERK1/2 increased significantly.These results inferred that β-cypermethrine could inhibit the synthesis of testosterone through increasing the activity of ERK1/2 to decrease the activity of P450c17 mRNA and level of StAR Protein.更多还原