【摘要】 从6个品种马的血液中提取基因组DNA,根据GenBank中已发表的人、鼠、雪貂的DRD4 cDNA基因保守区序列,设计3对特异性引物,另1对由文献所得,对6个品种马的DRD4基因分4段进行PCR扩增,经电泳检测,分别呈4条特异条带,将其分别克隆入pMD19-T载体中,进行序列测定并拼接序列,得到包括所有内含子的DRD4基因序列。分析结果表明,马DRD4基因含有4个外显子和3个内含子,所得序列与人、鼠和狗同源区比对,发现其同源性都达到了75%以上。对6个品种马DRD4基因序列比较发现,不同品种马之间,第1内含子和第3外显子存在一定的变异,包括转换、颠换、插入/缺失和VNTR。更多还原

【Abstract】 The genome DNA was extracted from the blood of six horse breeds including SH,BH,XN,WZ,TB,AI.Four pair of primers were designed based on the sequence of human,mouse and ferret DRD4 gene cDNA to amplify the corresponding horse gene.The PCR products were ligated into a linear plasmid pMD17,and sequenced respectively.These sequences were aligned to a conting to get most sequence of the horse DRD4 gene.The horse DRD4 gene has four exons and three introns,and share above 75% similarity with human,mouse and dog.And by comparing the DRD4 gene sequence of six breeds,some variations among the sequence of six breeds horse DRD4 gene were detected in intron1 and exon3.更多还原