【摘要】 研究体外定向诱导鸡胚精原干细胞(Spermatogonial stem cells,SSCs)向成骨细胞分化的能力。采用地塞米松+β-磷酸甘油钠+维生素C组成的诱导液对鸡胚精原干细胞进行诱导,利用Von Kossa’s法、改良的钙钴法及免疫组化I型胶原(CollagenⅠ)进行成骨细胞特性鉴定。结果表明:诱导后成骨细胞形成率达到82%,诱导后的细胞经Von Kossa’s染色可见细胞间布满黑色颗粒,提示有矿化基质沉积;改良钙钴法碱性磷酸酶染色胞浆呈深棕色或深黑色,免疫组化法染色细胞呈阳性。因此可以得出:鸡精原干细胞(SSCs)具有向成骨细胞分化的能力。更多还原

【Abstract】 Investigate the capability of spermatogonial stem cells(SSCs) differentiating to osteoblasts under different experimental conditions in vitro.In this study,the osteoblasts were induced from SSCs by desamethason,glycerol 2-phosphate disodium salt hydrate and vitamin C and were examined with Von Kossa’s stain,cytochemistry stain and immunohistochemistry stain(Collagen Ⅰ).The results indicated that the osteoblasts formed Von Kossa stain positive nodules,dark granules were found in the cell,suggesting the deposition of mineralized materials;the osteoblasts showed brown or deep dark by alkaline phosphatasepositive stain and showed CollagenⅠpositive with immunohistochemistry stain.And so,we may know that the Spermatogonial stem cells(SSCs) have the capability to be differentiated to osteoblasts in vitro.更多还原