【摘要】 研究骨骼肌中表达的miRNAs对深入探明骨骼肌生长发育的调控机制具有十分重要的意义。采用比较基因组学与生物信息学相结合的方法,从猪骨骼肌中克隆了猪miR-206的前体(Precursor),对该miRNA前体的二级结构及与其它物种序列的同源性进行了分析。RT-PCR结果显示,该前体miRNA在猪大多数组织中均有表达,在骨骼肌中表达量最高。生物信息学分析表明miR-206前体序列在不同物种间具有较高的保守性。更多还原

【Abstract】 MiRNAs play important roles in skeletal muscle development.In this study,the porcine miR-206 precursor is!cloned and sequenced from skeletal muscle through comparative genomics approach.The secondary structure was predicted.RT-PCR results indicated that the miR-206 precursor is expressed in most of the tissues and exhibited the highest expression level in skeletal muscle.Homology analysis revealed that pre-miR-206 is highly conserved among different species.更多还原