【摘要】 2个绵羊二核苷酸微卫星BM143和BMS2508的PCR扩增产物在非变性(中性)聚丙烯酰胺凝胶电泳(nPAGE)中形成滞后于主带的影子带。影子带比主带小2 bp。实际上主带由两部分组成:表明微卫星正确大小的PCR特异扩增的高浓度条带(特异扩增带)和比特异扩增带小2 bp的PCR非特异扩增的低浓度条带(非特异扩增带)。本试验初步证明影子带是微卫星的一条特异扩增带和一条非特异扩增带错配形成的异源双链。本研究结果表明,影子带的出现并不改变特异扩增带在nPAGE中的正常迁移率。更多还原

【Abstract】 The PCR products of two ovine dinucleotide microsatellites BM143 and BMS2508 were separated on nondenaturing polyacrylamide gel electrophoresis(nPAGE).Shadow bands were seen 2 bp below the main bands.Actually the main band was composed of two components: specifically a PCR-amplified high-density band that indicates the correct size of the microsatellite(specific band) and nonspecifically a PCR-amplified low-density band that is 2 bp shorter than the specific band(nonspecific band).The results of this study preliminarily demonstrated that the shadow band was heteroduplex DNA formed by a specific band and a nonspecific band. Appearance of shadow bands did not change the normal mobility of specific bands on nPAGE.更多还原