【摘要】 目的观察新鲜羊膜移植治疗兔角膜碱烧伤的组织学变化。方法21只(42眼)新西兰大白兔制作角膜碱烧伤模型;15只(30眼)为实验组,6只(12眼)为对照组;实验组行新鲜羊膜移植术,对照组不处理。分别取术后第1周、第2周、第4周的角膜进行光镜及电镜观察。结果术后第1周、第2周及第4周观察发现,对照组眼表面及角膜基质中有较多的炎性细胞浸润,上皮形状不规则,排列不整齐,第4周表现最明显;实验组炎性细胞浸润较少,上皮排列较整齐,显示新鲜羊膜有较强的抑制炎症并促进上皮生长的作用。通过透射电镜观察,发现新鲜羊膜移植组角膜上皮生长良好、表面有丰富的微绒毛、胶原纤维排列整齐,细胞间桥粒连接较多;对照组无上皮生长,胶原纤维排列紊乱。结论新鲜羊膜移植术在角膜碱烧伤治疗中有抑制炎症反应、促进上皮生长的作用,能够有效地促进角膜上皮的愈合及细胞之间的紧密连接。更多还原

【Abstract】 Objective To investigative the changes of corneal architecture after fresh amniotic membrane transplantation (AMT) for the treatment of corneal alkali burns.Methods Twenty-one(42 eyes) New Zealand rabbits with corneal alkali burns were divided into the fresh AMT group (30 eyes) and the control group (12 eyes) randomly.The changes of corneal architecture were observed by light microscope and transmission electron microscope respectively 1 week,2 weeks and 4 weeks after transplantation.Results Histological examination of corneas revealed that there were more infiltrating cells in corneal superficial epithelial and stroma layer in the control group compared to those in fresh AMT group 1 week,2 weeks and 4 weeks after transplantation,with the most significance 4 weeks.And the control group also showed that the arrangement of corneal epithelium was markedly irregular in size and shape than that in the fresh AMT group.Compared to the control group,the junctions between the corneal epithelial cells were more clear,the arrangement of corneal epithelium and collage were more regularly in the fresh AMT group.Conclusion The fresh AMT can effectively reduce inflammatory infiltration,promote epithelial healing and enhance tight junctions between cells.更多还原