【摘要】 实验探索了热带观赏植物大岩桐离体快繁的有效途径,以大岩桐叶片作为外植体,建立再生体系。各阶段的最佳培养基配比结果如下:初代培养基:MS+6-BA 1.0 mg/L+NAA 0.5 mg/L;增殖培养基:MS+6-BA 1.0 mg/L+NAA 0.05 mg/L;壮苗培养基:MS+6-BA 1.0 mg/L+NAA 0.8 mg/L;生根培养基:1/2MS,得到的组培苗经炼苗移栽后成活率高达100%。该体系的建立为其规模化生产及遗传改良提供了前提条件。更多还原

【Abstract】 Sinningia speciosa is a category of tropical ornamental plant.The purpose of the experiment is to explore the efficient means to swiftly reproduce it in vitro.In this paper,the leaves of Sinningia speciosa are used as explants,and the issue culture regeneration system is esatablished.The optimum medium for each stage can be illustrated as follows : Primary culture :MS + 6-BA 1.0 mg/L+NAA 0.5 mg/L;Multiplication culture :MS + 6-BA 1.0 mg/L + NAA 0.05 mg/L;Rejuvenescence medium: MS+6-BA 1.0 mg/L+NAA 0.8 mg/L;Enrooting medium:1/2MS,and after hardening and transplanting,the shoot can survive perfectly.更多还原