【摘要】 目的探讨青蒿琥酯对单侧输尿管梗阻(UUO)模型大鼠肾组织中结缔组织生长因子(CTGF)和α-平滑肌肌动蛋白(α-SMA)表达水平的的影响,初步探讨青蒿琥酯抗肾间质纤维化的作用机制。方法雄性W istar大鼠80只随机分为假手术组、UUO模型组、青蒿琥酯小剂量组、大剂量组和科素亚组,采用免疫组化半定量方法检测肾组织中CTGF和α-SM A的表达水平。结果UUO模型组和各治疗组中,术后3天肾间质即出现CTGF和α-SMA表达,术后14天表达最高;不同剂量青蒿琥酯组和科素亚组CTGF和-αSM A表达均低于UUO模型组,且大剂量组疗效优于小剂量组,而与科素亚组相似。结论青蒿琥酯可以降低肾间质中CTGF和α-SMA的表达,这可能是青蒿琥酯抗肾间质纤维化的作用机制之一。更多还原

【Abstract】 ObjectiveTo observe the expression of connective tissue growth factor(CTGF) and α-smooth muscle actin(α-SMA) in renal tissue of rat model of unilateral ureteral obstruction(UUO) treated with Artesunate,and investigate the mechanism of its antifibrotic effect on renal interstitial tissue.MethodsEighty male Wistar rats were randomly allocated into 5 groups,including sham-operated group,UUO group without any treatment,UUO groups treated with low-dose of Artesunate,high-dose of Artesunate or Losartan.The expression of CTGF and α-SMA in renal tissues was examined by sem-quantitative immunohistochemistry methods.ResultsExcept in the Sham-operated group,both CTGF and α-SMA were found in renal interstitial tissue since the 3rd day after UUO,and reached the peak levels on the 14th day.The expression of CTGF and α-SMA was higher in the UUO group without any treatment,which was suppressed in the groups with treatment.The reduction was not different between the Losartan group and the high-dose Artesunate group,whereas it was shown to be greater in the latter than in the low-dose of Artesunate group.ConclusionArtesunate decreases the expression of CTGF and α-SMA in renal interstitial tissue,which may explain one of the mechanisms of its antifibrotic effect.更多还原