【摘要】 建立了液相色谱-质谱联用(LC/MS)测定蜂王浆中土霉素、四环素、金霉素、强力霉素等四环素族抗生素残留的方法。样品用0.1mol/L Na2EDTA-Mcllvaine缓冲溶液提取,经过OasisHLB柱净化,然后采用LC/MS进行测定。本方法的加标回收率为65.0%～86.8%之间,相对标准偏差在5.53%～11.30%之间,对蜂王浆中土霉素、四环素的检出限小于10ng/g,金霉素、强力霉素的检出限小于20ng/g。更多还原

【Abstract】 Liquid chromatography-mass spectrometry method(LC -MS)for the determination of four tetracycline antibiotics including oxytetracycline, teracycline, chlortetracycline, and doxycycline in royal jelly are presented. The tetracy-cline antibiotic residues were extracted from royal jelly samples using a buffer containing 0.1mol/L Na2EDTA-Mcllvaine.The sample extracts were further purified and enriched by Oasis HLB.The recoveries of four tetracyclines ranged from 65.0 to 86. 8% in fortified blanks, the relative standard deviations were between 5.53 % and 11.30 %. The method detection limits of oxytetracycline and teracycline were <10ng/g, chlortetracycline and doxycycline were <20ng/g.更多还原