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液相色谱质谱法测定蜂王浆中四环素族抗生素残留

Determination of Tetracycline Antibiotic Residues in Royal Jelly by LC/MS

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【作者】 薛晓锋张金振赵静周金慧

【Author】 Xue Xiaofeng, Zhang Jinzhen, Zhao Jing, Zhou Jinhui ( Bee Products Quality Supervision and Testing Center, Ministry of Agriculture P.R.China, Beijing 100093, China )

【机构】 农业部蜂产品质量监督检测测试中心农业部蜂产品质量监督检测测试中心 北京100093北京100093

【摘要】 建立了液相色谱-质谱联用(LC/MS)测定蜂王浆中土霉素、四环素、金霉素、强力霉素等四环素族抗生素残留的方法。样品用0.1mol/L Na2EDTA-Mcllvaine缓冲溶液提取,经过OasisHLB柱净化,然后采用LC/MS进行测定。本方法的加标回收率为65.0%~86.8%之间,相对标准偏差在5.53%~11.30%之间,对蜂王浆中土霉素、四环素的检出限小于10ng/g,金霉素、强力霉素的检出限小于20ng/g。

【Abstract】 Liquid chromatography-mass spectrometry method(LC -MS)for the determination of four tetracycline antibiotics including oxytetracycline, teracycline, chlortetracycline, and doxycycline in royal jelly are presented. The tetracy-cline antibiotic residues were extracted from royal jelly samples using a buffer containing 0.1mol/L Na2EDTA-Mcllvaine.The sample extracts were further purified and enriched by Oasis HLB.The recoveries of four tetracyclines ranged from 65.0 to 86. 8% in fortified blanks, the relative standard deviations were between 5.53 % and 11.30 %. The method detection limits of oxytetracycline and teracycline were <10ng/g, chlortetracycline and doxycycline were <20ng/g.

【基金】 基金资助:2003~2004年度行业标准制订。
  • 【文献出处】 现代科学仪器 ,Modern Scientific Instruments , 编辑部邮箱 ,2007年01期
  • 【分类号】O657.72;S896.3
  • 【被引频次】24
  • 【下载频次】343
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