【摘要】 本文用紫外光谱、显微傅立叶变换红外光谱和圆二色光谱对聚阳离子海美溴铵(PB)和DNA之间的相互作用进行研究。以溴化乙锭作为与DNA结合的荧光探针,考察PB对其结合体系的影响。荧光分析结果显示,PB与DNA的相互作用可能对DNA二级结构的构型产生影响。IR光谱表明,PB通过磷酸基团和DNA碱基与DNA发生相互作用,DNA/PB复合物的形成引起DNA二级结构的构型变化,这一点也在CD光谱分析中得到验证。更多还原

【Abstract】 The interaction of polybrene(PB) with hsDNA has been studied by spectroscopic analysis,microscopic IR-and CD-spectroscopy.Using ethidium bromide(EB) as a fluorescent probe,the binding of PB with DNA was studied.The fluorescence analysis shows that the interaction of DNA with PB may have effect on the conformation of secondary structure of DNA.IR-spectra show that PB interacts with DNA through both the phosphate groups and the bases of DNA and the formation of DNA/PB complex causes the change of the conformation of the DNA secondary structure,which is further proved by CD-spectra.更多还原