【摘要】 [目的]研究神经生长因子(NGF)对大鼠胚胎脊髓神经细胞分化和增殖的作用。[方法]加入NGF到大鼠胚胎脊髓神经细胞进行培养,显微镜下计数存活脊髓神经细胞集落数及测定细胞相对蛋白质的含量。[结果]脊髓神经细胞胞体增大、突起延长且有丰富的神经纤维连结成网络状,细胞集落数增加,细胞相对蛋白质含量增加,显示出剂量—效应关系。[结论]一定剂量的NGF能促进大鼠脊髓神经细胞分化和增殖,增强其活性。更多还原

【Abstract】 [Objective]To study the effect of nerve growth factor(NGF)on differentiation and proliferation of the embryonic rat spinal cord neurons.[Methods]After NGF was added to embryonic spinal cord neurons from the embryonic rat,the number of survival spinal cord neurons was counted and relative protein content in the cells was tested by light microscopy.[Results]The cultured embryonic spinal cord neurons body were aggrandized,the cell protuberance was prolonged,plenty of neuron fiber formed net work,the number of survival spinal cord neurons and the relative protein content increased,which showed the dose-effect relation.[Conclusion]Certain concentration of NGF(20.0~80.0 ng/ml)can promote the differentiation and proliferation of embryonic spinal cord neurons,and enhance the activity of the neurons.更多还原