【摘要】 [目的]探寻一种无创伤性的早期诊断大肠癌的方法。[方法]采用聚合酶链反应—单链构象多态性(PCR—SSCP)技术检测大肠肿瘤病人粪便脱落细胞和组织APC基因突变并加以分析比较。[结果]肿瘤组织突变率为44%(19/43)。患者粪便脱落细胞APC基因的突变率为37.75%(16/43),腺瘤和大肠癌APC基因突变分别为25%(2/8)和40%(14/35)。组织粪便APC基因突变率相比差异无统计学意义(χ2检验P﹥0.05)。两者一致性检验结果kappa值为0.8449,一致性极好。正常组织APC基因未发现有突变。[结论]在粪便能够检测出APC基因突变,且与组织检测到的突变一致性极好,所以大肠肿瘤粪便的APC基因检测有望成为大肠肿瘤早期诊断和人群筛检的最优方案。更多还原

【Abstract】 [Objective]To probe into the feasibility of gene diagnosis screeing for colorectal carcinoma.[Methods]PCR-SSCP technique was used to detect APC gene mutation of tumor tissues and stools and their relationship and difference were analysed and compared.[Results]The mutation rate of tumor tissue was 44%(19/43). The mutation rate of epithelial cell shed from intestine in stool was 37.75%(15/13). The positive rate of adenomas and CRC were 25%(2/8)and 40%(14/35)respectively. There was no difference between the positive rate of the tumor tissues and stools by chi-square test(P﹥0.05).[Conclusions]APC gene mutation can be found in the stools,and the result is well consistent with that of tumor tissues detection. So the fecal gene detection is prospective to be the best method to diagnosis and screen out early CRC.更多还原