【摘要】 [目的]探讨大剂量经口纳米粒径二氧化钛(nano-TiO2)和三氧化二铝(nano-Al2O3)粉染毒对小鼠血清乳酸脱氢酶(LDH)和α-羟丁酸脱氢酶(α-HBDH)活力的影响。[方法]分别以5g/Kg体重剂量的微米(micro-TiO2)和nano-TiO2或-Al2O3粉给小鼠一次经口灌胃,14d后处死取血分离血清,分析LDH和α-HBDH活性变化。[结果]nano-Al2O3染毒小鼠血清LDH和α-HBDH活力比对照组明显降低(P﹤0.05),但与micro-Al2O3染毒组相比差异无统计学意义(P﹥0.05);nano-TiO2染毒小鼠血清LDH活力比对照组有明显升高(P﹤0.05),micro-TiO2染毒组小鼠血清LDH活力明显低于对照组和nano-TiO2染毒组(P﹤0.05);nano-TiO2染毒小鼠血清α-HBDH活力与micro-TiO2染毒组相比差异无统计学意义(P﹥0.05)。[结论]nano-TiO2和nano-Al2O3粉对小鼠血清LDH和α-HBDH活性的影响明显不同,nano-TiO2和nano-Al2O3粉对小鼠血清LDH和α-HBDH活性的影响与相应的micro-TiO2和micro-Al2O3有明显差别,血清LDH和α-HBDH可能不一定适合于纳米金属材料的生物毒性评估。更多还原

【Abstract】 [Objective]To explore the effect of excess nano-particle alumina oxide and titanium dioxide on serum Lactate dehydrogenase(LDH)and α-Hydroxybutyrate Dehydrogenase(α-HBDH)activity in mice.[Methods]The Mice were treated with micro-or nano-particle alumina oxide or titanium dioxide powder at a single oral dose of 5 g/Kg body weight. 14 days later,the mice were sacrificed and the serum was collected for LDH and α-HBDH analysis.[Results]The serum LDH and HBDH activity in Nano-Al2O3 group mice were lower significantly than that in control group but without obvious difference with that in micro-Al2O3 group mice. The serum LDH activity of nano-TiO2 group mice was significantly higher than that of control group mice,but there was no any difference in serum HBDH activity between micro-and nano-TiO2 group mice. The serum LDH activity in micro-TiO2 group mice decreased significantly than control group and nano-TiO2 group;there was no any difference in serum HBDH activity among the control,nano- and micro-TiO2 group mice.[Conclusions]There are significant different effects on serum LDH and α-HBDH between excess Nano-Al2O3 and Nano-TiO2 treated mice,excess nano-Al2O3 or nano-TiO2 has obvious different effect on serum LDH and α-HBDH than micro-Al2O3 or micro-TiO2,serum LDH and HBDH activity are probably not suitable for the evaluation on the biological injury and toxic of nano metal materials.更多还原