【摘要】 目的建立高效液相色谱测定十取代芦丁硫酸钠含量及有关物质。方法采用XDB-C8色谱柱,流动相:乙腈-25 mmol.L-1TBAB缓冲液(KH2PO410 mmol.L-1,三乙胺调节pH7.5)=48∶52,流速:1.0 mL.min-1,检测波长:λ=254 nm;柱温:25℃。结果在2.0~200.0μg.mL-1浓度范围内峰面积与浓度呈现良好的线性关系,回归方程为:A=12158.5C+43.63,相关系数r=0.999 9。日内精密度和日间精密度均小于3.5%。结论本法专属性良好,符合十取代芦丁硫酸钠的含量测定和有关物质检查的要求。更多还原

【Abstract】 OBJECTIVE To establish a HPLC method for determination of RDS and relative impurities.METHODS Agilent C8 column was used.The mobile phase consisted of acetonitrile and 25 mmol·L-1 phosphate buffer solution containing 20 mmol·L-1 TBAB(pH 7.5)(48∶52,v/v) with flow rate of 1.0 mL·min-1.The detection wavelength was set at 254 nm. RESULTS The linear range of carlibration curve was 2.0~200.0 μg·mL-1,A=12158.5C+43.63,r=0.999 9.The intra-day and inter-day RSD were less than 3.5%.CONCLUSION The proposed method was simple,precise and reproducible;the decomposed product can be separated as well.Therefore,the quality of RDS can be effectively controlled.更多还原