【摘要】 目的:温度敏感性磁性纳米颗粒即磁性纳米颗粒-Pluronic具有温度响应药物控制释放能力,并能穿透血脑屏障,实验检测其携带GM-1能力及在活体组织中药物释放能力,以及对脊髓损伤修复再生的影响。方法:动物实验于2006-09/2007-02在首都医科大学北京神经科学研究所完成。将20只SD大鼠制作急性完全横断脊髓损伤模型,随机分为4组:①治疗组:于脊髓断端间注射磁性纳米颗粒-Pluronic-GM-1100μL+纤维蛋白胶100μL。②对照组1:于脊髓断端间注射磁性纳米颗粒-Pluronic100μL+纤维蛋白胶100μL。③对照组2:于脊髓断端间注射纤维蛋白胶100μL。④空白组:单纯横断不予以任何治疗。4周后,应用免疫组织化学方法(NF和GFAP)结合图像分析方法,对脊髓横断处远端、近端进行神经纤维计数及胶质细胞网格框架结构定量分析,以了解神经再生情况。结果:20只大鼠均进入结果分析。①脊髓横断区神经纤维数量:治疗组近端、远端均高于其他3组(P<0.05),2个对照组高于空白组(P<0.05),对照组2高于对照组1(P<0.05)。②脊髓横断区胶质纤维面积比:治疗组近端、远端均高于其他3组(P<0.05),2个对照组近端明显高于空白组(P<0.05),对照组2远端高于空白组(P<0.05)。结论:磁性纳米颗粒-Pluronic具有药物携带及释放作用,其携带GM-1对脊髓损伤具有一定程度的修复及促进神经再生的作用。更多还原

【Abstract】 AIM: Magnetic Pluronic (MagPluronic) nanoparticles with their temperature sensitivity can control and release the temperature for drugs as a response, and penetrate blood-brain barrier. This study is designed to investigate the drug release capacity of ganglioside (GM)-1 loaded MagPluronic nanoparticles, and their effects on promoting regeneration of spinal cord injury. METHODS: The experiment was accomplished in Beijing Institute of Neuroscience, Capital Medical University from September 2006 to February 2007. Laminectomy was done in 20 adult SD rats for acute complete transection on the spinal cord, then those rats were divided into 4 groups at random: ①Treated group: Rats were implanted with 100 μL GM-1 loaded MagPluronic nanoparticles and 100 μL fibrin glue covering broken ends of spinal cord and filling the lesion gap.②Control group 1: Rats were implanted with 100 μL MagPluronic nanoparticles and 100 μL fibrin glue.③Control group 2: Rats were implanted 100 μL fibrin glue.④Blank group: Only transection was performed. Four weeks later, the nerve fiber count and the quantitative analysis of gridding frame structure of astrocytes were conducted by the means of immunohistochemistry and image analysis, so as to investigate the nerve regeneration of distal and proximal sites after spinal cord transection. RESULTS: All the 20 rats were involved in the result analysis.①The amount of never fibers in the distal and proximal sites after spinal cord transection: The amount was higher in treated group than in control groups and blank group (P < 0.05), higher in control groups than in blank group (P < 0.05), and also higher in control group 2 than in control group 1 (P < 0.05).②The ratio of gelatinous fibers area in spinal cord transection lesion: The ratio was the highest in the distal and proximal sites of treated group (P < 0.05), higher in the proximal sites of control groups than in blank group (P < 0.05), and also higher in control group 2 than in blank group (P < 0.05). CONCLUSION: MagPluronic nanoparticles have the ability of carrying and releasing drugs, so GM-1 loaded MagPluronic nanoparticles can promote repairs and nerve regenerations of the injured spinal cord.更多还原