【摘要】 目的:观察葛根素对乙醇所致睾丸损伤的保护作用,探讨其可能机制。方法:实验于2006-04-03/2007-02-20在四川大学人体解剖学教研室实验室进行。取SD成年雄性大鼠24只,随机分为3组:①乙醇组:给予白酒灌胃(乙醇体积分数为0.56),10mL/(kg·d)。②葛根素组:给予与乙醇组等量白酒灌胃后,立即腹腔注射葛根素62.5mg/(kg·d)。③对照组:给予10mL/(kg·d)生理盐水灌胃。给药连续3个生精周期共39d,于第40天麻醉后处死取睾丸,测定睾丸组织中超氧化物歧化酶活性及丙二醛含量;免疫组织化学法检测各组Bcl-2,Bax蛋白在生精细胞的表达情况;TUNEL法检测生精细胞的凋亡。结果:24只均进入结果分析。①乙醇组睾丸匀浆超氧化物歧化酶活性低于对照组(P<0.01),丙二醛含量高于对照组(P<0.01),葛根素组与对照组相比差异不显著(P>0.05)。②乙醇组平均每个生精小管断面中Bcl-2蛋白阳性细胞数和A值低于对照组(P<0.01),葛根素组与对照组比较差异不显著(P>0.05)。③乙醇组平均每个生精小管断面中Bax蛋白的阳性细胞数和A值高于对照组(P<0.01),但葛根素组与对照组比较差异不显著(P>0.05)。④乙醇组每个生精小管横切面中的凋亡细胞数目高于对照组(P<0.01),葛根素组与对照组比较差异不显著(P>0.05)。结论:乙醇通过氧化损伤作用使生精细胞发生凋亡,葛根素有对抗乙醇所致睾丸损伤的作用。更多还原

【Abstract】 AIM: To investigate the effect of puerarin on protecting against ethanol-induced injury in rat testis. METHODS: The experiment was carried out in the laboratory of Human Anatomy Department in Sichuan University from April 3rd 2006 to February 20th 2007. Twenty-four SD adult male rats were divided into three groups: ①Group A: The rats were lavaged with 10 mL/kg liquor of 56% alcohol dairy;②Group B: The rat was treated with 10 mL/kg liquor of 56% alcohol and 62.5 mg/kg puerarin by intraperitoneal injection;③Group C: The rat was treated with equal volume of NaCl. All the rats were treated for 39 days continuously in three spermatogenic cycles. At the 40th day, testis tissues were obtained after anesthesia to measure the contents of superoxide dismutase (SOD) and malondialdehyde (MDA); expressions of Bcl-2 and Bax in spermatogenic cells were checked by immunohistochemistry; Apoptosis of spermatogenic cells was determined by TUNEL. RESULTS: All of the 24 rats were involved in the result analysis.①SOD activity in group A was lower than that in group C (P < 0.01), but MDA was adverse (P < 0.01). There was no significant statistical difference between group B and group C (P > 0.05).②The expression of Bcl-2 and A value in seminiferous tubule section were lower in group A than in group C (P < 0.01), and there was no significant statistical difference between group B and group C (P > 0.05).③The expression of Bax and A value in seminiferous tubule section were higher in group A than in group C (P < 0.01), and there was no significant statistical difference between group B and group C (P > 0.05).④Apoptosis of spermatogenic cells in group A was higher than that of group C (P < 0.01), but there was no significant statistical difference between group B and group C (P > 0.05). CONCLUSION: Spermatogenic cells generate apoptosis by oxidative damage of alcohol. Puerarin can inhibit oxidative damage of didymus by alcohol.更多还原